Starch production rate throughout the experimental time course

Value 6.3 µmol glucose/gFW/hr Range: ±0.3 µmol glucose/gFW/hr
Organism Thale cress Arabidopsis thaliana
Reference Ma F, Jazmin LJ, Young JD, Allen DK. Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation. Proc Natl Acad Sci U S A. 2014 Nov 25 111(47):16967-72. doi: 10.1073/pnas.1319485111. p.16968 left column bottom paragraphPubMed ID25368168
Method Abstract: "[Investigators] performed in vivo isotopic labeling of Arabidopsis thaliana rosettes with (13)CO2 and estimated fluxes throughout leaf photosynthetic metabolism by INST-MFA [Isotopically nonstationary metabolic flux analysis]. Plants grown at 200 µmol m^(-2)s^(-1) light were compared with plants acclimated for 9 d at an irradiance of 500 µmol⋅m^(-2)⋅s^(-1)."
Comments P.16968 left column bottom paragraph: "Starch and Sucrose Measurements: Starch and sucrose, two significant products of leaf photosynthetic metabolism, were quantified to determine the times during the day that leaves exhibit pseudosteady-state metabolism. The amount of starch and sucrose per unit FW [fresh weight] were measured hourly from morning to midday. The leaves produced starch at a rate of 6.3 ± 0.3 µmol glucose/gFW/hr throughout the experimental time course (Fig. S1). The sucrose pool size did not change significantly during the same period, indicating that the biosynthetic and export rates were balanced. Therefore, plant leaves were isotopically labeled in the late morning."
Entered by Uri M
ID 113452