The number of GAGA transcription factor molecules per nucleus

Value 1e+6 Molecules/nucleus Range: Table - link Molecules/nucleus
Organism Fruit fly Drosophila melanogaster
Reference Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1PubMed ID22014521
Primary Source Wilkins RC, Lis JT. GAGA factor binding to DNA via a single trinucleotide sequence element. Nucleic Acids Res. 1998 Jun 1 26(11):2672-8.PubMed ID9592153
Method (primary source abstract:) "[Researchers] directly compare the affinity of GAF [GAGA transcription factor] for different sequence elements by immunoprecipitation and gel mobility shift analysis."
Comments P.617 left column bottom paragraph: "A survey of all credible published estimates that [Biggin] could find for four well studied species suggests that most animal transcription factors are expressed at 10,000–300,000 molecules per nucleus (Table 1). These estimates are further supported by [his] own experience and that of many other biochemists who have purified additional endogenous transcription factors with similar yields to the proteins in Table 1." P.620 right column bottom paragraph: "The data in Table 1 may be biased toward easily studied proteins, so the identification of transcription factors expressed at lower levels may identify better candidates for Coselective DNA Binding-based regulation." In Schneider cell line 2
Entered by Uri M
ID 106846