| Value |
50000
Molecules/nucleus
Range: Table - link Molecules/nucleus
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1PubMed ID22014521
|
| Primary Source |
Krause HM, Klemenz R, Gehring WJ. Expression, modification, and localization of the fushi tarazu protein in Drosophila embryos. Genes Dev. 1988 Aug2(8):1021-36.PubMed ID3049237
|
| Method |
(primary source abstract:) Analysis of embryonic ftz protein on Western blots allowed approximation of protein number in nucleus. |
| Comments |
P.617 left column bottom paragraph: "A survey of all credible published estimates that [Biggin] could find for four well studied species suggests that most animal transcription factors are expressed at 10,000–300,000 molecules per nucleus (Table 1). These estimates are further supported by [his] own experience and that of many other biochemists who have purified additional endogenous transcription factors with similar yields to the proteins in Table 1." P.620 right column bottom paragraph: "The data in Table 1 may be biased toward easily studied proteins, so the identification of transcription factors expressed at lower levels may identify better candidates for Coselective DNA Binding-based regulation." In Ftz expressing blastoderm nuclei. Note-primary source abstract and p.1024 right column top paragraph give ~20,000 rather than 50,000 copies/nucleus. |
| Entered by |
Uri M |
| ID |
106845 |