The number of Grainyhead transcription factor molecules per nucleus

Value 150000 Molecules/nucleus Range: Table - link Molecules/nucleus
Organism Fruit fly Drosophila melanogaster
Reference Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1PubMed ID22014521
Primary Source Harrison MM, Botchan MR, Cline TW. Grainyhead and Zelda compete for binding to the promoters of the earliest-expressed Drosophila genes. Dev Biol. 2010 Sep 15 345(2):248-55.PubMed ID20599892
Comments P.617 left column bottom paragraph: "A survey of all credible published estimates that [Biggin] could find for four well studied species suggests that most animal transcription factors are expressed at 10,000–300,000 molecules per nucleus (Table 1). These estimates are further supported by [his] own experience and that of many other biochemists who have purified additional endogenous transcription factors with similar yields to the proteins in Table 1." P.620 right column bottom paragraph: "The data in Table 1 may be biased toward easily studied proteins, so the identification of transcription factors expressed at lower levels may identify better candidates for Coselective DNA Binding-based regulation." In all blastoderm nuclei
Entered by Uri M
ID 106848