| Value |
20000
Molecules/nucleus
Range: Table - link Molecules/nucleus
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1PubMed ID22014521
|
| Primary Source |
Little SC, Tkacik G, Kneeland TB, Wieschaus EF, Gregor T. The formation of the Bicoid morphogen gradient requires protein movement from anteriorly localized mRNA. PLoS Biol. 2011 Mar9(3):e1000596.PubMed ID21390295
|
| Method |
(primary source abstract:) "[Researchers] adapt a novel method of fluorescent in situ hybridization to quantify the global spatio-temporal dynamics of bicoid mRNA particles." |
| Comments |
P.617 left column bottom paragraph: "A survey of all credible published estimates that [Biggin] could find for four well studied species suggests that most animal transcription factors are expressed at 10,000–300,000 molecules per nucleus (Table 1). These estimates are further supported by [his] own experience and that of many other biochemists who have purified additional endogenous transcription factors with similar yields to the proteins in Table 1." P.620 right column bottom paragraph: "The data in Table 1 may be biased toward easily studied proteins, so the identification of transcription factors expressed at lower levels may identify better candidates for Coselective DNA Binding-based regulation." Bcd measured in anterior blastoderm nuclei |
| Entered by |
Uri M |
| ID |
106843 |