Protein-to-phospholipid mass ratios of liver membranes used in SXS experiments

Range Table - link
Organism Rat Rattus norvegicus
Reference Mitra K, Ubarretxena-Belandia I, Taguchi T, Warren G, Engelman DM. Modulation of the bilayer thickness of exocytic pathway membranes by membrane proteins rather than cholesterol. Proc Natl Acad Sci U S A. 2004 Mar 23 101(12):4083-8 p. 4085 table 1 doi: 10.1073/pnas.0307332101PubMed ID15016920
Primary Source 21) Bergstrand A, Dallner G. Isolation of rough and smooth microsomes from rat liver by means of a commerically available centrifuge. Anal Biochem. 1969 Jun29(3):351-6 (22) Keenan TW, Morré DJ. Phospholipid class and fatty acid composition of golgi apparatus isolated from rat liver and comparison with other cell fractions. Biochemistry. 1970 Jan 6 9(1):19-25 (23) Meier PJ, Sztul ES, Reuben A, Boyer JL. Structural and functional polarity of canalicular and basolateral plasma membrane vesicles isolated in high yield from rat liver. J Cell Biol. 1984 Mar98(3):991-1000.PubMed ID4307201, 4312390, 6699096
Method (Ref:)Solution x-ray scattering (SXS). "SXS permits direct determination of the distance (d) between the highly electron-dense phosphate groups across the bilayer of membranes in solution (14, 15, 17). A limitation of this technique is that extramembranous protein domains and cell components containing highly electron-dense groups, such as ribosomes, can affect the quality of the scattering data (37)."
Comments "To establish the effect of membrane proteins on membrane thickness, [researchers] measured the average bilayer thickness of liposomes formed from total lipid extracts of each type of membrane. The protein, cholesterol, and phospholipid content of the reconstituted liposomes was analyzed, indicating that, on lipid extraction, all proteinaceous components were absent and that [they] were able to reproduce the cholesterol to phospholipid ratio of the original membranes (Table 1 and Fig. 4A)." Some data in table from primary sources. See notes under table. Numbers of primary sources correspond to those in article.
Entered by Uri M
ID 105818