Cholesterol depletion 1: Protein depletion 4-5 Å
||Mitra K, Ubarretxena-Belandia I, Taguchi T, Warren G, Engelman DM. Modulation of the bilayer thickness of exocytic pathway membranes by membrane proteins rather than cholesterol. Proc Natl Acad Sci U S A. 2004 Mar 23 101(12):4083-8 p. 4086 right column top paragraph (cholesterol depletion) bottom paragraph (protein depletion) doi: 10.1073/pnas.0307332101PubMed ID15016920
||Solution x-ray scattering (SXS). SXS permits direct determination of the distance (d)
between the highly electron-dense phosphate groups across the
bilayer of membranes in solution.
||Researchers show that removal
of membrane proteins from rat hepatocyte Golgi and
basolateral plasma membranes, and E. coli cell membranes, can
increase or decrease bilayer thickness by up to 4–5Å, depending on
the membrane studied, suggesting that membrane proteins can
significantly modulate the bilayer thickness of these membranes.