Mass of RNA in exponentially growing B/r cell with doubling time 100 min

Value 20 fg
Organism Bacteria Escherichia coli
Reference Bremer, H., Dennis, P. P. (1987) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 1st ed. chapter 96, Table 2 pp.1530-1 Table - link
Primary Source Nierlich DP. Regulation of ribonucleic acid synthesis in growing bacterial cells. II. Control over the composition of the newly made RNA. J Mol Biol. 1972 Dec 30 72(3):765-77. Table - link PubMed ID4573848
Method Calculated from data in leftmost column in table link in ref and according to average molecular mass of RNA nucleotide in E. coli of 324 Da (Primary source): (324[g per mole]/Avogadro's constant[nmber of dNTPs])X(number of RNA nucleotides per OD460)/(number of cells per OD460)=weight [g/cell]:(324/(6X10^23))X(4.3X10^16)/(11.7X10^8)=19.9X10^-15 g
Comments Out of cell dry mass of 148X10^-15 g at this doubling time. Reference table gives Protein/mass, RNA/mass, DNA/mass, cell no./mass, protein/genome, RNA/genome, origins/genome, protein/origin and more parameters at 5 different doubling times
Entered by Uri M
ID 104887