Mass of DNA in exponentially growing B/r cell with doubling time 100 min

Value 7.5 fg
Organism Bacteria Escherichia coli
Reference Bremer, H., Dennis, P. P. (1996) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed. chapter 97, Table 2 Table - link
Primary Source Churchward G, Estiva E, Bremer H. Growth rate-dependent control of chromosome replication initiation in Escherichia coli. J Bacteriol. 1981 Mar145(3):1232-8.PubMed ID7009573
Method DNA per mass was calculated from DNA per cell and cells per mass: GM(genome per mass)=GC(genome per cell)XCM(cell per mass) These calculated values closely agree with direct measurements of DNA per mass (Fig 1 in primary source), using the colorimetric diphenylamine reaction with E. coli DNA as a calibration standard and assuming 1 A260 unit of E. coli DNA to correspond to 2.86X10^13 kbp (for a GC content of 0.5) and the E. coli genome to be 4,700 kbp. Calculated from data in table link in ref and according to average molecular mass of DNA base pair of 618 Da: (618[g per mole base pairs]/Avogadro's constant[number of base pairs per mole])X(genome equivalents per cell)X(number of base pairs per genome)=weight [g/cell]:(618/(6X10^23))X(1.56)X(4.7X10^9)=7.57X10^-15 g/cell
Comments Out of cell dry mass of 148X10^-15 g at this doubling time. See BNID 104878, 104887. Reference table gives Protein/mass, RNA/mass, DNA/mass, cell no./mass, protein/genome, RNA/genome, origins/genome, protein/origin and more parameters at 5 different doubling times
Entered by Uri M
ID 104906