Mass of protein in exponentially growing B/r cell with doubling time 100 min

Value 100 fg
Organism Bacteria Escherichia coli
Reference Bremer, H., Dennis, P. P. (1987) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 1st ed. chapter 96, Table 2 pp.1530-1 Table - link
Primary Source Spahr PF. Amino acid composition of ribosomes from Escherichia coli. J Mol Biol. 1962 May4:395-406. Table - PubMed ID13915520
Method Calculated from data in leftmost column in table link in ref and according to average molecular mass of amino acid in E. coli protein of 108 Da (Primary source): (108[g per mole]/Avogadro's constant[number of aa residues per mole]) X(number of aa residues per OD460)/(number of cells per OD460)=weight [g/cell]:(108/(6X10^23))X(6.5X10^17)/(11.7X10^8)=10^-13 g
Comments Out of cell dry mass of 148X10^-15 g at this doubling time. Note-according to calculation from Primary source table link average amino acid molecular mass is 109 Da, see BNID 104877. Reference table gives Protein/mass, RNA/mass, DNA/mass, cell no./mass, protein/genome, RNA/genome, origins/genome, protein/origin and more parameters at 5 different doubling times
Entered by Uri M
ID 104878