Mass of protein in exponentially growing B/r cell with doubling time 40 min

Value 234 fg
Organism Bacteria Escherichia coli
Reference Bremer, H., Dennis, P. P. (1996) Modulation of chemical composition and other parameters of the cell by growth rate. Neidhardt, et al. eds. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed. chapter 96, Table 2 pp.1530-1 Table - link
Primary Source Spahr PF. Amino acid composition of ribosomes from Escherichia coli. J Mol Biol. 1962 May4:395-406. Table - link PubMed ID13915520
Method See note 'h' beneath table: Calculated from data in table link in ref and according to average molecular mass of amino acid in E. coli protein of 108 Da (Primary source): (108[g per mole]/Avogadro's constant[aa residues per mole]) X(number of aa residues per OD460)/(number of cells per OD460)=weight [g/cell]:(108/(6X10^23))X(5.2X10^17)/(4.0X10^8)=234X10^-15 g
Comments Out of cell dry mass of 433X10^-15 g at this doubling time. Note-according to calculation from Primary source table link average amino acid molecular mass is 109 Da, see BNID 104877. Reference table gives Protein/mass, RNA/mass, DNA/mass, cell no./mass, protein/genome, RNA/genome, origins/genome, protein/origin and more parameters at 5 different doubling times
Entered by Uri M
ID 104879