Enrichment in free amino acids, other metabolites, and in amino acids in protein in Col-0 growing in a 8 h photoperiod

Range Table - link
Organism Thale cress Arabidopsis thaliana
Reference Ishihara H, Obata T, Sulpice R, Fernie AR, Stitt M. Quantifying protein synthesis and degradation in Arabidopsis by dynamic 13CO2 labeling and analysis of enrichment in individual amino acids in their free pools and in protein. Plant Physiol. 2015 May168(1):74-93. doi: 10.1104/pp.15.00209. Supplementary data p.34/38 table S1PubMed ID25810096
Method Supplementary data p.33/38 note above table: "(A) Estimated [13C]-enrichment (%) of free amino acids and other metabolites. (B) Estimated [13C]-enrichment (%) of amino acids in total protein. Col-0 was grown in for 18 days in an 8 h light 16 h dark photoregime, transferred to the labeling chamber for 3 days in the same photoregime and then, starting at dawn, pulsed with 13CO2 for 24 h followed by a 4 day chase. Plants were harvested just before the start of the pulse, after 4 h and 8 h light, as well as at dusk and dawn during the first day of the chase, at dawn at the end of the 4 day chase. Four samples of 5 plants were harvested at each time point. The sampling times are given at the top of each column as hours after the start of pulse at dawn. The full data set including information about all measure isotopamers is provided in Supplemental Data Set S1, and plots of the labeling kinetics of individual isotopamers are provided in Figure 2 and Supplemental Figure S2. The results are given as plots shows the μ± S.D. of the four biological replicates."
Comments P.76 right column 2nd paragraph: "[Investigators] were able to quantify enrichment in 16 free amino acids (Fig. 1 Supplemental Table S1A). The temporal kinetics of enrichment varies between amino acids and was often slow and incomplete. During the pulse, enrichment of individual amino acids ranged between 83% and 11% at the end of day and between 71% and 15% at the end of night. During the chase, enrichment decreased rapidly for some amino acids and slowly for others. This underlines the importance of information about enrichment in free amino acid pools for accurate estimation of the rate of protein synthesis." Col-0=Columbia-0 plant
Entered by Uri M
ID 112888