13CO2 enrichment (%) of metabolites

Range Table - link %
Organism Thale cress Arabidopsis thaliana
Reference Szecowka M et al., Metabolic fluxes in an illuminated Arabidopsis rosette. Plant Cell. 2013 Feb25(2):694-714. doi: 10.1105/tpc.112.106989. Supplemental data p.12 Supplemental table 2PubMed ID23444331
Method P.695 right column 3rd paragraph:"In this study, [investigators] used a custom-designed labeling chamber to carry out short-term 13CO2 labeling of intact Arabidopsis rosettes under ambient steady state conditions. Several complementary analytical platforms were applied, including GC-TOF-MS [gas-chromatography-time-of-flight-mass spectrometry] and two recently developed liquid chromatography–tandem mass spectrometry (LC-MS/MS) platforms (Lunn et al., 2006 Arrivault et al., 2009), allowing quantitative determination and 13C enrichment calculation of 40 metabolites from the CBC [Calvin-Benson cycle], Suc and starch synthesis, glycolysis, amino acid and organic acid metabolism, as well as trehalose-6-phosphate (Tre6P) metabolism."
Comments P.702 left column 2nd paragraph:"The 12C isotopomer decay of Tre6P was slow and almost linear over the time course of the experiment (Figure 2A see Supplemental Figure 3A online), and, after 60 min of labeling, the unlabeled form still represented the predominant Tre6P isotopomer (∼40%). Tre6P showed mild 13C enrichment, with up to 21% at 60 min (Figures 2B and 2C). Trehalose also showed only mild 13C enrichment (i.e., up to 9, 18, and 14% at 10, 20, and 60 min, respectively) (Figures 2B and 2C see Supplemental Table 2 online). As the trehalose pool is 370-fold smaller than the Suc pool and is much less weakly labeled, it is only a very minor product. Based on their enrichment, Tre6P and trehalose clustered in the same group (Figure 1). Labeling of myo-inositol was very slow (see Supplemental Figure 6A online), with 13C enrichment of ∼3% after 60 min of labeling (see Supplemental Table 2 online)." See note beneath table.
Entered by Uri M
ID 112098