Metabolic flux distributions determined using 13C-tracers in mammalian iBMK cell, yeast & E. coli

Range Table - link
Organism Various
Reference Park JO et al., Metabolite concentrations, fluxes and free energies imply efficient enzyme usage. Nat Chem Biol. 2016 May 2. doi: 10.1038/nchembio.2077. Supplementary Information pp.2-3 Supplementary Table 1PubMed ID27159581
Method Abstract: "Here [investigators] use isotope labeling to measure absolute metabolite concentrations and fluxes in Escherichia coli, yeast and a mammalian cell line. [They] then integrate this information to obtain a unified set of concentrations and ΔG for each organism."
Comments P.2 right column top paragraph: "Together with nutrient uptake and waste excretion data, the labeling data were sufficient to determine pseudo-steady-state net fluxes throughout central metabolism by isotopomer and flux balancing. Results of all of the tracer experiments were integrated into a single unified flux set for each organism (Fig. 2a and Supplementary Table 1)." P.5 right column bottom paragraph: "In addition to the methodology for measuring ΔG, a key contribution of this work is a coherent systems-level set of fluxes, free energies and metabolite concentrations for E. coli, yeast and the mammalian cell line iBMK (Figs. 2–5 and Supplementary Tables 1, 4 and 5)." iBMK=Immortalized Baby Mouse Kidney Epithelial Cells
Entered by Uri M
ID 112690