Flux of glucose-derived two-carbon units into the TCA [tricarboxylic acid] cycle consumed the following fraction of pyruvate:

Range in E. coli 24%: in mammalian iBMK cell 13%: in yeast 2% % of pyruvate made
Organism Various
Reference Park JO et al., Metabolite concentrations, fluxes and free energies imply efficient enzyme usage. Nat Chem Biol. 2016 May 2. doi: 10.1038/nchembio.2077. p.2 right column 2nd paragraphPubMed ID27159581
Method Abstract: "Here [investigators] use isotope labeling to measure absolute metabolite concentrations and fluxes in Escherichia coli, yeast and a mammalian cell line. [They] then integrate this information to obtain a unified set of concentrations and ΔG for each organism."
Comments P.2 right column 2nd paragraph: "Flux of glucose-derived two-carbon units into the TCA cycle consumed 24% of pyruvate made in E. coli but only 13% in the mammalian iBMK cells and 2% in yeast, which had minimal α-ketoglutarate dehydrogenase flux, with malate dehydrogenase operating net in reverse. Malic enzyme flux was substantial in yeast and mammalian cells but not E. coli. Overall, fluxes were more similar in the two eukaryotic cell types than across the two microbes (Fig. 2b)." iBMK=Immortalized Baby Mouse Kidney Epithelial Cells
Entered by Uri M
ID 112685