Net flux through the oxidative pentose phosphate pathway as fraction of glucose uptake

Range in E. coli 20%: in yeast 3%: in mammalian iBMK cell 2% % of glucose uptake
Organism Various
Reference Park JO et al., Metabolite concentrations, fluxes and free energies imply efficient enzyme usage. Nat Chem Biol. 2016 May 2. doi: 10.1038/nchembio.2077. p.2 right column 2nd paragraphPubMed ID27159581
Method Abstract: "Here [investigators] use isotope labeling to measure absolute metabolite concentrations and fluxes in Escherichia coli, yeast and a mammalian cell line. [They] then integrate this information to obtain a unified set of concentrations and ΔG for each organism."
Comments P.2 right column 2nd paragraph: "Substantial differences in net fluxes were observed across the three organisms. The absolute glucose uptake rate, normalized to cell volume, was about 30-fold lower in the mammalian iBMK cells than in E. coli or yeast. Although the largest intracellular flux in each organism was glycolysis, they differed in their utilization of the pentose phosphate pathway and the tricarboxylic acid (TCA) cycle. Net flux through the oxidative pentose phosphate pathway was 20% of glucose uptake in E. coli but only 3% and 2% in yeast and mammalian iBMK cells, respectively." iBMK=Immortalized Baby Mouse Kidney Epithelial Cells
Entered by Uri M
ID 112684