Duration of pause of RNA polymerase on gene

Value 4 min
Organism Mammalian tissue culture cell
Reference Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, Singer RH. In vivo dynamics of RNA polymerase II transcription. Nat Struct Mol Biol. 2007 Sep14(9):796-806.PubMed ID17676063
Method a method for the in vivo labeling of mRNA transcripts containing a series of repeated stem-loops (from phage MS2), which are specifically bound by an MS2 coat protein fused to green fluorescent protein (GFP). The assay consists of a human cell line harboring a gene array into which these stem-loops have been integrated
Entered by Uri M
ID 104586