|Mammalian tissue culture cell
|Hirschberg K et al., Kinetic analysis of secretory protein traffic and characterization of golgi to plasma membrane transport intermediates in living cells. J Cell Biol. 1998 Dec 14 143(6):1485-503. abstract right column, p.1486 right column top paragraph, p.1495 right column top paragraph & p.1501 left column 4th paragraphPubMed ID9852146
|Abstract: "Quantitative time-lapse imaging data of single cells expressing the transmembrane protein, vesicular stomatitis virus ts045 G protein fused to green fluorescent protein (VSVG-GFP), were used for kinetic modeling of protein traffic through the various compartments of the secretory pathway. A series of first order rate laws was sufficient to accurately describe VSVG-GFP transport, and provided compartment residence times and rate constants for transport into and out of the Golgi complex and delivery to the plasma membrane."
|Abstract: "Large pleiomorphic tubular structures, rather than small vesicles, were found to be the primary vehicles for Golgi to plasma membrane transport of VSVG-GFP. These structures budded as entire domains from the Golgi complex and underwent dynamic shape changes as they moved along microtubule tracks to the cell periphery. They carried up to 10,000 VSVG-GFP molecules [BNID 112597] and had a mean life time in COS cells of 3.8 min." P.1486 right column top paragraph: "Post-Golgi intermediates containing VSVG–GFP were large, irregularly-shaped structures that budded as a whole from the Golgi complex and moved along microtubules to the cell periphery without intersecting other membrane transport pathways. Quantitative kinetic modeling techniques revealed that these structures have a mean life time of 3.8 min within cells and are the major vehicles for VSVG–GFP delivery to the cell surface."