Summary of the expression state of genes measured in study and compared to literature

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Zenklusen D, Larson DR, Singer RH. Single-RNA counting reveals alternative modes of gene expression in yeast.Nat Struct Mol Biol. 2008 Dec15(12):1263-71. Supplemental table 4PubMed ID19011635
Primary Source Holstege FC et al., Dissecting the regulatory circuitry of a eukaryotic genome. Cell. 1998 Nov 25 95(5):717-28. & Ghaemmaghami, S. et al. Global analysis of protein expression in yeast. Nature. 2003. 425(6959) pp.737-41.PubMed ID9845373, 14562106
Method " in situ hybridization (FISH) approach that detects single mRNA molecules, mRNA abundance and transcriptional activity within single Saccharomyces cerevisiae cells." See fig. 8a: Synthesis time was plotted against the length of the gene. The slope of the line gives (polymerase speed)^-1
Comments "As shown in Supplementary Table 4 online, the genes used in this study show a three- to six-fold higher expression than that determined previously (ref 12, Holstege et al, Cell 1998 pubmed 9845373, report a value of 15000 mRNA transcripts per cell). This would correct the number of transcripts to around 60,000 mRNAs per cell and indicates that the yeast transcriptome is more active than initially thought." See BNID 104312
Entered by Uri M
ID 111032