Number of mRNA transcripts per cell

Range ~60,000 copies/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Zenklusen D, Larson DR, Singer RH. Single-RNA counting reveals alternative modes of gene expression in yeast.Nat Struct Mol Biol. 2008 Dec15(12):1263-71. p.1269 right column top paragraphPubMed ID19011635
Method " in situ hybridization (FISH) approach that detects single mRNA molecules, mRNA abundance and transcriptional activity within single Saccharomyces cerevisiae cells." "To achieve single-transcript resolution, [researchers] adapted a FISH technique previously described in mammalian cells [ref 22 PMID 9554849]."
Comments "As shown in Supplementary Table 4 online, the genes used in this study show a three- to six-fold higher expression than that determined previously (ref 12, Holstege et al, Cell 1998 pubmed 9845373, report a value of 15000 mRNA transcripts per cell). This would correct the number of transcripts to around 60,000 mRNAs per cell and indicates that the yeast transcriptome is more active than initially thought." See BNID 104312
Entered by Uri M
ID 103023