Number of polyadenylated mRNA transcripts

Range ~15,000 Copies/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference von der Haar T. A quantitative estimation of the global translational activity in logarithmically growing yeast cells. BMC Syst Biol. 2008 Oct 162:87 p.8/14 right column 2nd paragraphPubMed ID18925958
Primary Source Hereford LM, Rosbash M. Number and distribution of polyadenylated RNA sequences in yeast. Cell. 1977 Mar10(3):453-62. p.457 note "f" beneath table 1PubMed ID321129
Method (P.457 of primary source note "f" beneath table 1:)“‘Copies per cell’ is calculated with the following assumptions. The size of the genome is 9x10^9daltons (Bicknell and Doublas, 1970). The RNA/DNA ratio is 50:1 (Hartwell, 1970). The percentage of poly(A)-containing RNA is 1.5 (estimated from the difference if the Crot1/2 values of Figures 5 and 8). The average size of yeast mRNA is 1500 nucleotides. There are therefore approximately 15,000 mRNA molecules per cell. Copies per cell=15,000x(%cDNA)/(number of sequences).”
Comments "Although the total mass of cellular RNA cannot be calculated in the same way as proteins because non-coding mRNA regions for each gene contribute to the molecular weight but are not accurately known, the total number of mRNAs in the dataset can easily be calculated as about 12,200, with 95% confidence limits between 6,100 and 18,300 mRNAs per cell [BNID 102988]. This compares to experimental estimates of about 15,000 poly(A) tailed RNAs per cell generated experimentally [primary source]. In two important aspects, the curated dataset thus approaches estimates from available experimental data." See BNID 103023
Entered by Uri M
ID 104312