Diffusion coefficients of telomeres in the nucleus of human osteosarcoma U2OS cells

Value 1.8e-4 µm^2/sec
Organism Human Homo sapiens
Reference Görisch SM, Lichter P, Rippe K. Mobility of multi-subunit complexes in the nucleus: accessibility and dynamics of chromatin subcompartments.Histochem Cell Biol. 2005 Mar123(3):217-28 Table - link PubMed ID15830242
Primary Source Molenaar C, Wiesmeijer K, Verwoerd NP, Khazen S, Eils R, Tanke HJ, Dirks RW. Visualizing telomere dynamics in living mammalian cells using PNA probes. EMBO J. 2003 Dec 15 22(24):6631-41.PubMed ID14657034
Method (Primary source) Using peptide nucleic acid (PNA) probes, researchers studied the dynamic behavior of telomeric DNA repeats in living human osteosarcoma U2OS cells. A fluorescent cy3-labeled PNA probe was introduced in living cells by glass bead loading and was shown to specifically associate with telomeric DNA shortly afterwards. Telomere dynamics were imaged for several hours using digital fluorescence microscopy.
Comments Table gives corral size 'rc'-The size of the region in which a given particle or the chromatin locus can translocate its centre of mass during an observation time of up to a few minutes, defined by a circle with radius rc, and the diffusion coefficient within the corral. Corral radius=230 nm. Value observed for the majority of telomeres. A~10% fraction of telomeres showed a higher mobility with a diffusion coefficient of D=5.8×10^-4 µm^2/sec
Entered by Uri M
ID 104286