Diffusion coefficient of 10kDa dextran through diffusion channel of nuclear pore complex NPC

Value 76 µm^2/sec
Organism African clawed frog Xenopus laevis
Reference Keminer O, Peters R. Permeability of single nuclear pores. Biophys J. 1999 Jul77(1):217-28 Table - link PubMed ID10388751
Method optical single transporter recording (OSTR), for optically recording the activity of single transporters in membrane patches. Cells or organelles are firmly attached to isoporous filters, and the transport across membrane patches spanning filter pores is measured by confocal laser scanning microphotolysis (SCAMP) (Wedekind et al., 1994), the amalgamation of microphotolysis and laser scanning microscopy.
Comments Stokes radius of this protein is 2.82 nm. Number of measurements n=258. Table gives Diffusion coefficients and other parameters for diffusion of dextrans of various sizes across npc
Entered by Uri M
ID 104255