HeLa cell volume

Range 4400-5000 Table - link µm^3
Organism Human Homo sapiens
Reference Cohen LS, Studzinski GP. Correlation between cell enlargement and nucleic acid and protein content of HeLa cells in unbalanced growth produced by inhibitors of DNA synthesis. J Cell Physiol. 1967 Jun69(3):331-9. p.336 table 3PubMed ID4230858
Method Abstract: "HeLa cells in monolayer cultures were treated with the following inhibitors of DNA synthesis: mitomycin C, nitrogen mustard, fluorodeoxyuridine, hydroxyurea, arabinofuranosylcytosine and high concentrations of thymidine. The concentration of each inhibitor used was, in most cases, just sufficient to arrest cell multiplication and all produced unbalanced growth in the sense that the synthesis of RNA and protein were only partially inhibited while DNA synthesis stopped." P.332 right column top paragraph: "Three days were allowed for the cells to adhere to the Petri dishes and to enter the logarithmic phase of growth." P.332 right column bottom paragraph: "Cell number was obtained with a Coulter counter, while cell sizing was performed with the automatic particle size distribution analyser Model J Electronic Co., Hialeah, Fla."
Comments P.336 left column 2nd paragraph: "The increase in cell volume following the treatment with the antimetabolites assessed by three independent methods is shown in table 3." Please note-BNID 103725 gives HeLa cell volume range of 1,200-4,290µm^3. See BNID 105879
Entered by Uri M
ID 103719