Number of clonogenic cells in the adult anterior pituitary gland

Range ≈1000 clonogenic cells (representing ≈0.1–0.2% of the cells in the gland)
Organism Mouse Mus musculus
Reference Gleiberman AS et al., Genetic approaches identify adult pituitary stem cells. Proc Natl Acad Sci U S A. 2008 Apr 29 105(17):6332-7. doi: 10.1073/pnas.0801644105 p.6334 right column bottom paragraphPubMed ID18436641
Method P.6332 right column 2nd paragraph: "Nestin-Expressing Cells of the Adult Anterior Pituitary: To identify stem cells of the anterior pituitary, [investigators] used transgenic mice expressing GFP driven by regulatory elements of the nestin gene (ref 17)."
Comments P.6334 right column bottom paragraph: "Nestin-GFP Cells Generate Pituitary Lineages in Vitro: To extend this in vivo evidence of the presence of multipotent stem cells in the anterior pituitary gland, [investigators] developed a protocol for isolation and cultivation of nestin-GFP-expressing cells from the adult pituitary [Fig. 3 and supporting information (SI) Fig. S1]. These cells can give rise to exponentially growing colonies. There are ≈1,000 clonogenic cells in the adult anterior pituitary gland, representing ≈0.1–0.2% of the cells in the gland. These clonogenic cells can undergo at least 25 divisions, suggesting that they may have stem cell-like properties." Wikipedia: "A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells."
Entered by Uri M
ID 115297