Concentration of standard proteins

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Raue U, Oellerer S, Rospert S. Association of protein biogenesis factors at the yeast ribosomal tunnel exit is affected by the translational status and nascent polypeptide sequence. J Biol Chem. 2007 Mar 16282(11):7809-16 Supplementary table 1PubMed ID17229726
Method The concentration of each purified protein was determined by two independent methods. The amount of proteins applied to generate standard curves was calculated based on mean of the two values. Bradford-assay (Bio-Rad), DC protein-assay (Bio- Rad), and BCA-assay (Sigma) were performed according to the manufacturers’ manual with bovine serum albumin as a standard. The concentration of His6-Nat1 was determined with the DC proteinassay (Bio-Rad), which can be performed in the presence of detergent. The concentrations of His6- Asc1, His6-Ard1 and His6-Ssb1 were calculated from UV-absorption at 280 nm using molar extinction coefficients at 280 nm: e(His6-Asc1) = 71056 M-1 cm-1, e(His6- Ard1) = 23506 M-1 cm-1, and e(His6- Ssb1) = 19099 M-1 cm-1. Absorption at 280 nm could not be applied as a method for protein quantification when purification was performed under denaturing conditions, as urea interfered with measurements.
Entered by Uri M
ID 104308