| Range |
50-1300000 proteins/cell
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
P Picotti, A Kuemmel, R Costenoble, H Lam, D Campbell, LN Mueller, M Heinemann, E Deutsch, U Sauer, B Domon, and R Aebersold, Analysis of the S. cerevisiae metabolic network by targeted proteomics 2008 link |
| Primary Source |
Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS. Global analysis of protein expression in yeast. Nature. 2003 Oct 16 425(6959):737-41PubMed ID14562106
|
| Method |
(Primary source) To facilitate global protein analyses, researchers have created a Saccharomyces cerevisiae fusion library where each open reading frame is tagged with a high-affinity epitope and expressed from its natural chromosomal location. Through immunodetection of the common tag, they obtain a census of proteins expressed during log-phase growth and measurements of their absolute levels. |
| Comments |
Equivalent to ~1nM-30uM.
Primary source finds that the abundance of proteins ranges from fewer than 50 to more than 10^6 molecules per cell. |
| Entered by |
Uri M |
| ID |
104150 |