Minimal generation time on Luria Bertani medium

Value 18.1 min Range: ±0.52 min
Organism Bacteria Escherichia coli
Reference Reshes G, Vanounou S, Fishov I, Feingold M. Timing the start of division in E. coli: a single-cell study. Phys Biol. 2008 Nov 7 5(4):46001 p.2 right column 3rd paragraphPubMed ID18997273
Method Abstract: "[Researchers] monitor the shape dynamics of individual E. coli cells using time-lapse microscopy together with accurate image analysis. This allows measuring the dynamics of single-cell parameters throughout the cell cycle."
Comments P.2 right column 3rd paragraph: "[Researchers] find that up to five generations, the growth of N is exponential. In LB, the average doubling time for the 30 cells was 18.10 ± 0.52 min which is consistent with the generation time measured by monitoring OD600 of the corresponding liquid culture, 22.27 ± 0.69 min. The corresponding times for cells grown in M9-glucose were 38.38 ± 1.10 min and 48.64 ± 1.61 min, respectively. Although the difference between the generation times is significant (about 20% for both LB and M9-glucose), the faster growth rate on agar indicates that the solid support does not restrict cell growth." For similar minimal time see BNID 103514, and for various doubling times see BNID 102533, 103391, 102096, 104331, 104332, 101905 (left column of table link)
Entered by Uri M
ID 104331