Generation time with and without amino acids in the medium

Range with amino acids 26.4±7.2 without amino acids 46.8±17.0 min
Organism Bacteria Escherichia coli
Reference Ullman G, Wallden M, Marklund EG, Mahmutovic A, Razinkov I, Elf J. High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking. Philos Trans R Soc Lond B Biol Sci. 2012 Dec 24 368(1611):20120025. doi: 10.1098/rstb.2012.0025. p.4 right column bottom paragraphPubMed ID23267179
Method P.2 left column top paragraph:"In this study, [investigators] report on a method combining microfluidics, single-molecule fluorescence microscopy and automated image analysis, enabling the study of the expression and super-resolution localization of low copy number transcription factors throughout thousands of bacterial lifespans per experiment. To illustrate the performance of the method, [they] quantify the dynamics of synthesis and intracellular localization of the lactose repressor by monitoring LacI–Venus expressed from its native promoter in live E. coli cells. [They] compare these observations with those obtained under identical conditions for cells expressing the reporter construct Tsr–Venus from the lactose permease gene, lacY, of the lactose operon."
Comments p.4 right column bottom paragraph:"The generation time defines the growth rate of exponentially growing cells and is often used as an indicator of the health or fitness. [Investigators] compare cells grown with and without amino acids in the medium (figure 3a, red and blue, respectively) and observe average generation times of 26.4 ± 7.2 and 46.8 ± 17.0 min, respectively."
Entered by Uri M
ID 112060