| Range |
with amino acids 26.4±7.2 without amino acids 46.8±17.0 min
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Ullman G, Wallden M, Marklund EG, Mahmutovic A, Razinkov I, Elf J. High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking. Philos Trans R Soc Lond B Biol Sci. 2012 Dec 24 368(1611):20120025. doi: 10.1098/rstb.2012.0025. p.4 right column bottom paragraphPubMed ID23267179
|
| Method |
P.2 left column top paragraph:"In this study, [investigators] report on a method combining microfluidics, single-molecule fluorescence microscopy and automated image analysis, enabling the study of the expression and super-resolution localization of low copy number transcription factors throughout thousands of bacterial lifespans per experiment. To illustrate the performance of the method, [they] quantify the dynamics of synthesis and intracellular localization of the lactose repressor by monitoring LacI–Venus expressed from its native promoter in live E. coli cells. [They] compare these observations with those obtained under identical conditions for cells expressing the reporter construct Tsr–Venus from the lactose permease gene, lacY, of the lactose operon." |
| Comments |
p.4 right column bottom paragraph:"The generation time defines the growth rate of exponentially growing cells and is often used as an indicator of the health or fitness. [Investigators] compare cells grown with and without amino acids in the medium (figure 3a, red and blue, respectively) and observe average generation times of 26.4 ± 7.2 and 46.8 ± 17.0 min, respectively." |
| Entered by |
Uri M |
| ID |
112060 |