Growth energetics during glucose-limited continuous cultures

Range Table - link
Organism Bacteria Escherichia coli
Reference Kayser A, Weber J, Hecht V, Rinas U. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. I. Growth-rate-dependent metabolic efficiency at steady state. Microbiology. 2005 Mar151(Pt 3):693-706. DOI: 10.1099/mic.0.27481-0 p.698 table 3PubMed ID15758216
Method P.694 left column 4th paragraph: "In this study, the effect of growth rate on catabolic and anabolic fluxes and energetic efficiency of the E. coli K-12 strain TG1 growing in aerobic glucose-limited continuous culture was examined. Moreover, the energetic state of the cells was analysed by measurement of the intracellular adenosine nucleotide pool."
Comments P.698 right column top paragraph: "The values of Y[X/ATP] were calculated by adjusting their values to reach the experimentally determined biomass yield Y[X/Glucose] (Table 3) using the detailed stoichiometric model (see Appendix) constructed for estimation of the intracellular carbon flux distribution. Thus, based on the glucose uptake rate, the intracellular fluxes were calculated by linear programming with ‘maximize growth’ as the objective function, keeping the energetic parameters P/O and mATP constant and adjusting the third energetic parameter Y[X/ATP] (present in the biomass formation reaction, r97) to be compatible with the experimentally determined biomass yield Y[X/Glucose] (Table 3). The determination of Y[X/ATP] at different dilution rates using the detailed stoichiometric model revealed increasing values of Y[X/ATP] with increasing dilution rates (Table 3), indicating more energy-efficient biomass formation at higher growth rates, i.e. less ATP consumption for biomass generation, presumably caused by less futile cycling, leaks and growth-associated maintenance." See caption above table
Entered by Uri M
ID 113372