Concentrations of biomass, glucose, acetate and ammonium and volumetric CTR (carbon dioxide transfer rate) and OTR (oxygen transfer rate) during steady-state growth

Range Table - link
Organism Bacteria Escherichia coli
Reference Kayser A, Weber J, Hecht V, Rinas U. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. I. Growth-rate-dependent metabolic efficiency at steady state. Microbiology. 2005 Mar151(Pt 3):693-706. DOI: 10.1099/mic.0.27481-0 p.696 table 1PubMed ID15758216
Method P.694 left column 4th paragraph: "In this study, the effect of growth rate on catabolic and anabolic fluxes and energetic efficiency of the E. coli K-12 strain TG1 growing in aerobic glucose-limited continuous culture was examined. Moreover, the energetic state of the cells was analysed by measurement of the intracellular adenosine nucleotide pool."
Comments P.695 right column bottom paragraph: "Aerobic glucose-limited continuous steady-state cultures with dilution rates ranging from D=0.044 to 0.415 h^-1 were used to determine the growth parameters of E. coli TG1. The concentrations of biomass, glucose and acetate and the volumetric CTR as a function of the dilution rate are shown in Fig. 1 (also summarized in Table 1). At dilution rates above 0.4 h^-1, the concentrations of glucose and acetate increased considerably concomitant with a strong decrease in the biomass concentration and the volumetric CTR, clearly showing that growth at these dilution rates was close to the wash-out point. All data obtained from steady-state cultures were tested for consistency by applying a reactor mass balance for the elements carbon and nitrogen (Table 1). The nitrogen recovery was always above 97 %, a satisfactory carbon recovery, however, was only obtained for dilution rates up to 0.4 h^-1, which prompted us to omit the data of the two steady states at dilution rates above 0.4 h^-1 (shaded area in Fig. 1) from further calculations."
Entered by Uri M
ID 113370