Yield characteristics of E. coli B growing in glucose-limited anaerobic continuous culture at 25 and 30˚C

Range Table - link
Organism Bacteria Escherichia coli
Reference Mainzer SE, Hempfling WP. Effects of growth temperature on yield and maintenance during glucose-limited continuous culture of Escherichia coli. J Bacteriol. 1976 Apr126(1):251-6. p.252 table 1PubMed ID770423
Method p.251 right column 2nd paragraph:"E. coli B was maintained as described previously (ref 7). The composition of the growth medium and descriptions of the continuous culture apparatus and the techniques employed in continuous culture were described previously (ref 8). Methods for the estimation of oxygen consumption, glucose utilization, acetate production, and bacterial dry weight were also given previously (ref 8). When it was necessary to carry out continuous culture at temperatures below the ambient value in the laboratory, the entire chemostat apparatus was placed in a constant-temperature room maintained at 12 C, and the temperature of the vessel was then adjusted and regulated as described previously (ref 8)."
Comments p.252 left column:"The results of experiments carried out at two temperatures other than 37 C are given in Table 1. YATP was calculated from the amount of glucose metabolized and of acetate produced (ref 8). When the YATP values (Table 1) are plotted versus the dilution rate D (Table 1), a rectangular hyperbola results (ref 12). Two different linear transformations of the data give the results shown in Table 1." See notes beneath table.
Entered by Uri M
ID 111762