| Comments |
P.5 left column top paragraph: "2.3.3. Interferometry: A newly developed alternative method to tube pulling and micropipette aspiration for measuring
in-plane membrane tension uses a non-invasive high-speed interferometric approach to detect membrane fluctuations (refs [33, 34] and figure 1). Fluctuations can be related to the membrane tension, but as with membrane tethers and micropipette aspiration, if the cytoskeleton is attached to the membrane, the fluctuations also reflect the acto-myosin dynamics of the cell. When cytoskeleton is destroyed however, this technique gives comparable values to those obtained by tube pulling on
cell blebs [primary source 35] (table 1), and thus is a promising complement
to existing membrane tension measurement techniques, with the advantage that unlike tether pulling and micropipette aspiration, the cell is not physically manipulated during the
measurement." |