Half-life of pre-mRNA in mouse embryonic fibroblasts (3T3)

Range Table - link min
Organism Mouse Mus musculus
Reference Hao S, Baltimore D. RNA splicing regulates the temporal order of TNF-induced gene expression. Proc Natl Acad Sci U S A. 2013 Jul 16 110(29):11934-9. doi: 10.1073/pnas.1309990110. Supporting Information p.13 table S4PubMed ID23812748
Method Abstract: "When cells are induced to express inflammatory genes by treatment with TNF [Tumor necrosis factor], the mRNAs for the induced genes appear in three distinct waves, defining gene groups I, II, and III, or early, intermediate, and late genes. To examine the basis for these different kinetic classes, [investigators] have developed a PCR-based procedure to distinguish pre-mRNAs from mRNAs."
Comments P.11937 left column bottom paragraph: "There were some differences in pre-mRNA half-life measured at different introns of individual genes (Table S3), possibly suggesting that introns may be spliced independently, but this observation requires further study. Pre-mRNA in general have longer half-life in mouse fibroblasts than in mouse macrophage (comparing Tables S3 and S4). [Investigators] saw a similar phenomenon for the mRNA half-life between these two types of cells (ref 9). However, in all measurements, the differences between early genes and the other two groups remained evident (Tables S3 and S4, Fig. 4A, and Fig. S4 A–D). This consistency among cell types suggests that the differences in RNA splicing reflect a gene intrinsic property. Thus, intrinsically longer RNA splicing times appear the best explanation for the delayed mRNA appearance for group II and III genes." For methods and notes, see table S3 link
Entered by Uri M
ID 112439