Amino acid composition of the protein as measured (mol %)

Range Table - link mol %
Organism Budding yeast Saccharomyces cerevisiae
Reference Lange HC, Heijnen JJ. Statistical reconciliation of the elemental and molecular biomass composition of Saccharomyces cerevisiae. Biotechnol Bioeng. 2001 Nov 5 75(3):334-44. p.339 table IVPubMed ID11590606
Method Abstract: "A systematic mathematical procedure capable of detecting the presence of a gross error in the measurements and of reconciling connected data sets by using the maximum likelihood principle is applied to the biomass composition data of yeast. The biomass composition of Saccharomyces cerevisiae grown in a chemostat under glucose limitation was analyzed for its elemental and for its molecular composition. Both descriptions initially resulted in conflicting results concerning the elemental composition, molecular weight, and degrees of reduction. The application of the statistical reconciliation method, based on elemental balances and equality relations, is used to obtain a consistent biomass composition."
Comments P.338 right column 3rd paragraph: "Measurements of the Molecular Biomass Composition: The relative abundance of each amino acid did not vary between the different cultures and the average measured value (Table IV) was taken to determine the elemental protein composition as CH1.581N0.275O0.318S0.003. Most measured values were within ±10% of the ones reported by Oura (1972), Chistyakova et al. (1982), and Schulze (1995). A distinction between free amino acids and protein was not attempted. Trials with pure proteins exhibited variations in total recovery due to different susceptibility to hydrolyzation since the ratios of amino acids were in agreement with the literature values. On average, 0.82 ± 5% of the protein was recovered thus, measurements of the biomass protein content were adjusted accordingly."
Entered by Uri M
ID 112800