Intracellular copper concentration

Range 1.3±0.2x10^6 atoms/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Meera R Raja, Scott R Waterman, Jin Qiu , Reiner Bleher, Peter R Williamson and Thomas V. O'Halloran, A copper hyperaccumulation phenotype correlates with pathogenesis in Cryptococcus neoformans, Metallomics. 2013 Apr5(4):363-71. doi: 10.1039/c3mt20220h. p.365 right column 2nd paragraphPubMed ID23511945
Primary Source [34] J. L. Wolford. Zinc Localization and Quantitation in Specialized Cells and Tissues. Northwestern University, Evanston, IL, 2006.
Comments P.365 right column 2nd paragraph: "Copper quota of C. neoformans is higher than that of S. cerevisiae: [Investigators] next tested whether high levels of copper accumulation was a typical feature of fungal cell growth and compared the copper content in C. neoformans and the non-pathogenic yeast Saccharomyces cerevisiae. To minimize variances due to different media compositions, both of the cell types were grown in YPD media, which contains ∼400 nM copper as analyzed by ICP-MS ([Inductively coupled plasma mass spectrometry], data not shown). While S. cerevisiae contains 1.3 ± 0.2 × 10^6 atoms of copper per cell (primary source) the C. neoformans cells accumulated almost three times that amount of copper per cell, i.e. 3.7 ± 0.7 × 10^6 (Fig. 3). This pathogen clearly accumulates more copper than brewers yeast when grown in culture. To further elucidate whether C. neoformans also accumulates copper to high levels when growing within the cell host and test the copper homeostasis pathways involved, [they] next examined fungal isolates from mouse models." See BNID 108833
Entered by Uri M
ID 108840