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||Park JO et al., Metabolite concentrations, fluxes and free energies imply efficient enzyme usage. Nat Chem Biol. 2016 May 2. doi: 10.1038/nchembio.2077. Supplementary Information p.4 Supplementary Table 2PubMed ID27159581
||Abstract: "Here [investigators] use isotope labeling to measure absolute metabolite concentrations and fluxes in Escherichia coli, yeast and a mammalian cell line. [They] then integrate this information to obtain a unified set of concentrations and ΔG for each organism."
||P.2 right column 3rd paragraph: "In addition to determining net fluxes, an in-house algorithm was developed to quantify forward-to-backward flux ratios and their confidence intervals and associated ΔG (Online Methods). The ability to obtain informative flux ratios depends on the backwards reaction producing a distinctive labeling pattern. The tracers employed enabled such direct measurement of forward-to-backward flux ratios for selected reactions in the pentose phosphate pathway, glycolysis, the TCA cycle and folate-mediated serine-glycine interconversion (Fig. 3 and Supplementary Table 2)." iBMK=Immortalized Baby Mouse Kidney Epithelial Cells