Number of copies of HIV-1 RNA surrounding infected activated and resting CD4+ T cells in SIV infected animal

Range surrounding infected activated cells 191±89: surrounding infected resting cells 34±12 copies of HIV-1 RNA
Organism macaque
Reference De Boer RJ, Ribeiro RM, Perelson AS. Current estimates for HIV-1 production imply rapid viral clearance in lymphoid tissues. PLoS Comput Biol. 2010 Sep 2 6(9):e1000906. doi: 10.1371/journal.pcbi.1000906. p.2 left column bottom paragraphPubMed ID20824126
Primary Source [23] Reilly C, Wietgrefe S, Sedgewick G, Haase A. Determination of simian immunodeficiency virus production by infected activated and resting cells. AIDS. 2007 Jan 11 21(2):163-8.PubMed ID17197806
Comments P.2 left column bottom paragraph: "One can also attempt to measure burst size by directly imaging the extracellular viral particles surrounding an infected cell [primary source]. Using this method, Reilly et al. [primary source] found 191±89 and 34±12 copies of HIV-1 RNA surrounding infected activated and resting CD4+ T cells, respectively, in the lymphoid tissue of acutely SIV-infected rhesus macaques. They then fitted a five parameter model to this data, with three of the parameters describing the rate of viral production as a function of time since infection (see Methods), and the remaining parameters describing the rate of exponential decay of cells producing virus, and the rate of loss of viral particles. Using this model, they estimated that the half-life of virus located around CD4+ T cells producing virus in lymphoid tissue was approximately three hours. However, this half-life combined diffusion of virus out of the local area and true virion clearance [primary source]."
Entered by Uri M
ID 112647