| Range |
40 - 80 min
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Tenaillon O, Skurnik D, Picard B, Denamur E. The population genetics of commensal Escherichia coli. Nat Rev Microbiol. 2010 Mar8(3):207-17. doi: 10.1038/nrmicro2298 p.208 left column top paragraphPubMed ID20157339
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| Primary Source |
[14] Poulsen LK, Lan F, Kristensen CS, Hobolth P, Molin S, Krogfelt KA. Spatial distribution of Escherichia coli in the mouse large intestine inferred from rRNA in situ hybridization. Infect Immun. 1994 Nov62(11):5191-4PubMed ID7927805
|
| Method |
Primary source abstract: "Fluorescent oligonucleotide probes targeting rRNA were used to develop an in situ hybridization technique by which the spatial distribution of Escherichia coli in the large intestines of streptomycin-treated mice was determined. Single E. coli cells were identified in thin frozen sections from the large intestines by the use of a probe specific for E. coli 23S rRNA. Furthermore, the total bacterial population was visualized with an rRNA probe targeting the domain Bacteria. By this technique, all E. coli cells were seen embedded in the mucosal material overlying the epithelial cells of the large intestine, and no direct attachment to the epithelium was observed." |
| Comments |
P.208 left column top paragraph: "Although the concentrations of these sugars in the intestine are low [ref 17], E. coli maximizes its growth by using micro-aerobic and anaerobic respiration in the intestine [ref 18]. This results in a 30 minute generation time in vitro on intestinal mucus [BNID 116914 ref 19] compared with 40–80 minutes in the intestines of streptomycin-treated mice, in which the cells in the luminal content are static [primary source], and 120 minutes when the mice are 'conventionalized' by removing the streptomycin and feeding them with mouse caecal content [ref 20]. This change in growth rate in the presence of other species illustrates that E. coli competes with those other species." |
| Entered by |
Uri M |
| ID |
116915 |