| Range |
≈1000 clonogenic cells (representing ≈0.1–0.2% of the cells in the gland)
|
| Organism |
Mouse Mus musculus |
| Reference |
Gleiberman AS et al., Genetic approaches identify adult pituitary stem cells. Proc Natl Acad Sci U S A. 2008 Apr 29 105(17):6332-7. doi: 10.1073/pnas.0801644105 p.6334 right column bottom paragraphPubMed ID18436641
|
| Method |
P.6332 right column 2nd paragraph: "Nestin-Expressing Cells of the Adult Anterior Pituitary: To identify
stem cells of the anterior pituitary, [investigators] used transgenic mice
expressing GFP driven by regulatory elements of the nestin gene
(ref 17)." |
| Comments |
P.6334 right column bottom paragraph: "Nestin-GFP Cells Generate Pituitary Lineages in Vitro: To extend this in vivo evidence of the presence of multipotent stem cells in the anterior pituitary gland, [investigators] developed a protocol for isolation and cultivation of nestin-GFP-expressing cells from the adult pituitary [Fig. 3 and supporting information (SI) Fig. S1]. These cells can give rise to exponentially growing colonies. There are ≈1,000 clonogenic cells in the adult anterior pituitary gland, representing ≈0.1–0.2% of the cells in the gland. These clonogenic cells can undergo at least 25 divisions, suggesting that they may have stem cell-like properties." Wikipedia: "A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells." |
| Entered by |
Uri M |
| ID |
115297 |