| Range |
Schizosaccharomyces pombe 2.34±0.18: Schizosaccharomyces japonicus 1.8±0.22 hours
|
| Organism |
Yeast |
| Reference |
Makarova M et al., Temporal Regulation of Lipin Activity Diverged to Account for Differences in Mitotic Programs. Curr Biol. 2016 Jan 25 26(2):237-43. doi: 10.1016/j.cub.2015.11.061 p.240 right column top paragraphPubMed ID26774782
|
| Method |
Abstract: "Eukaryotes remodel the nucleus during mitosis using a variety of mechanisms that differ in the timing and the extent of nuclear envelope (NE) breakdown. Here, [investigators] probe the principles enabling this functional diversity by exploiting the natural divergence in NE management strategies between the related fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus [refs 1-3]. [Investigators] show that inactivation of Ned1, the phosphatidic acid phosphatase of the lipin family, by CDK [Cyclin-dependent kinases] phosphorylation is both necessary and sufficient to promote NE expansion required for "closed" mitosis in S. pombe." |
| Comments |
P.240 right column top paragraph: "Indeed, the population-doubling times were comparable between the lipin-swapped and the wild-type cultures (2.34 ± 0.18 versus 2.31 ± 0.11 hr for S. pombe wild-type and Ned1[S.j.] and 1.8 ± 0.22 versus 1.84 ± 0.13 hr for S. japonicus wild-type and Ned1[S.p.]). The fact that the two lipins could substitute for each other when expressed in a heterologous system indicates that the regulatory modes rather than the lipin protein properties diverged between the two fission yeasts." |
| Entered by |
Uri M |
| ID |
114003 |