Population doubling time of 17 HUES (Human Embryonic Stem cells)

Range 11 HUES ~24-48hrs: 3 HUES 60-72hrs: 3 HUES N.D Table - link hrs
Organism Human Homo sapiens
Reference Cowan CA et al., Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med. 2004 Mar 25 350(13):1353-6. DOI:10.1056/NEJMsr040330 Supplementary Appendix p.4 Supplementary Table 1.PubMed ID14999088
Method P.1353 left column top paragraph: "To promote further research with human embryonic stem cells, [investigators] sought to derive and characterize more fully cell lines that meet strict criteria for ease of manipulation, including enzymatic passage with trypsin, streamlined freezing and thawing procedures, well-defined culture mediums, and straightforward methods for in vitro differentiation. [They] report the derivation and characterization of 17 additional human embryonic stem cell lines."
Comments P.1354 right column bottom paragraph: "The population doubling time for HUES1, HUES2, HUES4, HUES6 through HUES9, and HUES13 through HUES16 is approximately 24 to 48 hours (Figure 2 and Table 1 in Supplementary Appendix 1). Three cell lines, HUES3, HUES5, and HUES10, have slightly longer population doubling times — between 60 and 72 hours. [Investigators] found that low-passage human embryonic stem cells had longer population doubling times (approximately 150 hours) and that with additional passages, the population doubling times shortened and stabilized (Table 1 and Figure 3 in Supplementary Appendix 1, and additional data not shown)." Population doubling times of HUES11, HUES12, and HUES17 were not determined (N.D)
Entered by Uri M
ID 112761