Colony numbers and population doubling time in mesenchymal stem cells derived from different sources

Range Table - link
Organism Sheep Ovis aries
Reference Heidari B et al., Comparison of proliferative and multilineage differentiation potential of sheep mesenchymal stem cells derived from bone marrow, liver, and adipose tissue. Avicenna J Med Biotechnol. 2013 Apr5(2):104-17. p.7/14 table 2PubMed ID23799179
Method Abstract: "The primary cell cultures were prepared form tissue samples obtained from sheep 30-35 day fetus. Passage-3 cells were plated either at varying cell densities or different serum concentrations for a week. The Population Doubling Time (PDT), growth curves, and Colony Forming Unit (CFU) of MSCs (Mesenchymal Stem Cells) was determined. The stemness and trilineage differentiation potential of MSCs were analyzed by using molecullar and cytochemical staining approaches. The data was analyzed through one way ANOVA using SigmaStat (ver. 2)."
Comments P.6 right column 4th & 5th paragraphs: "Assessment of PDT in both MSCs lines was revealed no difference in PDT between two sources of MSCs when the cells plated at 10^4 cells/cm^2 in a culture medium supplemented with 10% FBS (Fetal bovine serum, Table 2). Concerning the colonogenic capacity of MSCs, no difference was observed in the number of fibroblast shape colonies (each more than 20 cells) following 100 cells seeding in 10 cm petri dish after 9 days between bone marrow and liver derived MSCs (Table 2, Figure 2)."
Entered by Uri M
ID 112658