| Range |
Grx 2 ≤1%: Grx3 0.5%: Trx1 especially at the stationary phase 0.3% %
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Potamitou A, Holmgren A, Vlamis-Gardikas A. Protein levels of Escherichia coli thioredoxins and glutaredoxins and their relation to null mutants, growth phase, and function. J Biol Chem. 2002 May 24 277(21):18561-7. DOI: 10.1074/jbc.M201225200 link p.18565 left column bottom paragraph PubMed ID11893749
|
| Method |
Abstract: "Levels of Escherichia coli thioredoxin 1 (Trx1), Trx2, glutaredoxin 1 (Grx1), Grx2, and Grx3 have been determined by novel sensitive sandwich enzyme-linked immunosorbent assay." |
| Comments |
P.18565 left column bottom paragraph: "General Redoxin Levels—The striking finding is that Grx2, an atypical large glutaredoxin with structure similar to glutathione S-transferase (primary sources), exists in the cell in amounts as high up as 1% of total protein. Grx3 was also found at relatively high levels (0.5%), as was Trx1 especially at the stationary phase (0.3%). The best characterized Grx1 comprises at best (300–600 ng/mg) one-tenth of the amount of Grx2 and Grx3 found under normal conditions-this is worth pointing out because Grx2 and Grx3 still have a relatively unknown function(s). The levels of the redoxins under normal growth suggest that Trx1 and Grx2 are stationary phase proteins, whereas Grx1 is a protein of the exponential phase." |
| Entered by |
Uri M |
| ID |
113257 |