Levels of thioredoxins 1 and 2 and glutaredoxins 1, 2, and 3 in a wild type strain (DHB4)

Range Table - link ng/mg total protein
Organism Bacteria Escherichia coli
Reference Potamitou A, Holmgren A, Vlamis-Gardikas A. Protein levels of Escherichia coli thioredoxins and glutaredoxins and their relation to null mutants, growth phase, and function. J Biol Chem. 2002 May 24 277(21):18561-7. DOI: 10.1074/jbc.M201225200 link p.18563 table III PubMed ID11893749
Method Abstract: "Levels of Escherichia coli thioredoxin 1 (Trx1), Trx2, glutaredoxin 1 (Grx1), Grx2, and Grx3 have been determined by novel sensitive sandwich enzyme-linked immunosorbent assay." Caption on top of table III: "Wild type strain (DHB4) was grown for 5 days in LB medium, and samples were taken at different time points. Redoxin levels were determined with sandwich ELISA. Values represent means of duplicates."
Comments P.18563 right column 2nd paragraph: "Levels of Thioredoxins 1 and 2 and Glutaredoxins 1, 2, and 3 in a Wild Type Strain—Wild type (DHB4) strain was grown in 1 liter of culture for 5 days in a 3-liter Erlenmeyer flask in LB media (Table III). The levels of Grx3 and Trx2 were stable (~4.5 and 0.2 µg/mg, respectively), Grx1 levels decreased slightly (from 0.6 to 0.2 µg/mg), whereas those of Trx1 increased slightly (from 2.0 to 3.0 µg/mg). The levels of Grx2 increased during late exponential phase and were stable at late stationary phase. In late stationary phase the levels of Grx2 (8 µg/mg) were ~3-fold higher than during exponential phase (2.5 µg/mg)."
Entered by Uri M
ID 113256