Levels of thioredoxins and glutaredoxins in wild type (DHB4) strain grown to exponential phase in LB medium (A600 ~0.8)

Range Grx1 ~200: Grx2 ~3,200: Grx3 ~4,400: Trx1 ~1,900: Trx2 ~50 ng/mg total protein
Organism Bacteria Escherichia coli
Reference Potamitou A, Holmgren A, Vlamis-Gardikas A. Protein levels of Escherichia coli thioredoxins and glutaredoxins and their relation to null mutants, growth phase, and function. J Biol Chem. 2002 May 24 277(21):18561-7. DOI: 10.1074/jbc.M201225200 link p.18565 figure 2 PubMed ID11893749
Method Abstract: "Levels of Escherichia coli thioredoxin 1 (Trx1), Trx2, glutaredoxin 1 (Grx1), Grx2, and Grx3 have been determined by novel sensitive sandwich enzyme-linked immunosorbent assay." Values extracted visually from figure 2
Comments P.18564 left column 2nd paragraph: "Changes in the Thioredoxin and Glutaredoxin Levels after Treatment with hydrogen Peroxide or Mercaptoethanol—To examine the regulation of different redoxins in reducing and oxidizing environments, cells were treated with hydrogen peroxide or mercaptoethanol. Grx1 levels increased up to 5-fold in cells treated with hydrogen peroxide, whereas Grx2 was downregulated under the same treatment but was up-regulated under mercaptoethanol treatment (Fig. 2). Grx3 responded in the same way as Grx2 but with much lower levels of change. Trx1 and Trx2 levels changed as did those of Grx1 but with much smaller amplitude. Hydrogen peroxide and mercaptoethanol treatment of katEkatG minus strains gave the same pattern of changes as described above but with the redoxin levels changing much more dramatically (Fig. 3)."
Entered by Uri M
ID 113255