Estimated thioredoxin reducing potential

Range -0.26 to -0.28 V
Organism Bacteria Escherichia coli
Reference Hwang, C., Sinskey, A. J., and Lodish, H. F. (1992). Oxidized redox state of glutathione in the endoplasmic reticulum. Science 257, 1496–1502 p.1499 left column top paragraphPubMed ID1523409
Primary Source [4] Gilbert HF. Molecular and cellular aspects of thiol-disulfide exchange. Adv Enzymol Relat Areas Mol Biol. 1990 63 :69-172.PubMed ID2407068
Method P.1499 left column top paragraph: "...calculated from the reduced state of E. coli thioredoxin."
Comments P.1498 right column: "These analyses explain why Escherichia coli cannot properly fold recombinant proteins with disulfide bonds, typical GSH/GSSG ratios for E. coli are in the range of 50:1 to 200:1 (ref 20), which is in agreement with the estimated redox potential of ~-0.26 to -0.28 V calculated from the redox state of E. coli thioredoxin (primary source)."
Entered by Avi Flamholz
ID 113978