Range |
Table - link days^-1
|
Organism |
bacteria |
Reference |
Marc Troussellier, Marc Bouvy, Claude courties, Christine dupuy, Variation of carbon content among bacterial species under starvation condition, Aquatic microbial ecology, Vol 13 :113-119. 1997 link p.117 table 3 |
Method |
P.114 right column 2nd & 3rd paragraphs: "At the same time, a subsample was filtered onto a black Nuclepore filter (0.2µm pore size) which was stored at 5˚C in the dark, and examined, within 48 h, with an epifluorescence microscope (Olympus BH with a 100×UVFL iris objective) to determine size of DAPI stained bacteria. Photographs (Kodak Tmax ASA 400) were taken from 10 randomly selected fields, then projected onto a digitizer tablet (final magnification, ×5000). Dimensions of 50 cells per sample were measured with a computer-assisted digitizer. Cell volume (V) was calculated assuming that the shape of bacteria was spherical or cylindrical with hemispheric ends: V=(πw^2/4)(l-w/3), where w is the width and l is the length of the cell (Bratbak 1985). Calibration was performed with microphotographs of a slide micrometer (Zeiss) and microspheres (Polysciences, Warrington, PA) of 0.51 and 0.75µm diameter." |
Comments |
P.115 left column 2nd paragraph: "Carbon content per cellular volume unit (Cv): In contrast, the amount of carbon per cellular volume unit (Cv) exhibited a significant increase during incubation for each strain (Figs. 1 & 2). The slopes of the linear regression model between Cv values and time showed that, except for Alteromonas rubra, marine strains have a higher Cv increase rate than non-marine strains (Table 3). At the end of the starvation period (28 d), there was a factor of 9 between the lowest (68.84, Escherichia coli) and the highest (609.52, Vibrio fischeri) CV values." |
Entered by |
Uri M |
ID |
112934 |