Carbon per cell in volume range 0.036 to 0.073µm^3

Range (Lee and Fuhrman 1987) 20 fg C/cell: (present study) 13 - 19 fg C/cell
Organism Bacteria Escherichia coli
Reference Meinhard Simon, Farooq Azam, Protein content and protein synthesis rates of planktonic marine bacteria. Marine ecology progress series. Oldendorf, 1989 Vol 51 pp.201-213 p.209 table 5 and p.210 left column 4th paragraph
Primary Source Lee S, Fuhrman JA. Relationships between Biovolume and Biomass of Naturally Derived Marine Bacterioplankton. Appl Environ Microbiol. 1987 Jun53(6):1298-303PubMed ID16347362
Method Primary source Lee and Fuhrman abstract: "In the present study, natural bacterioplankton assemblages were freshly collected, passed through 0.6-mum-pore-size Nuclepore filters to remove larger particulate materials, and diluted for growth in 0.22-mum-pore-size Millipore filter-sterilized unenriched seawater."
Comments P.210 left column 4th paragraph: "[Investigators’] results are consistent with, and explain, recent findings of very high C:cell volume ratios determined from carbon measurements in bacterial cultures or in size-fractionated natural bacterial assemblages: 354 mg C/ml (Bjørnsen 1986), 380 mg C/ml (primary source Lee and Fuhrman, 1987) and 560 mg C/ml (Bratbak 1985). Lee & Fuhrman (primary source) also found that 0.036 to 0.073 µm^3 cells uniformly contained 20 fg C/cell. [Investigators’] calculated carbon values for cells in this volume range [precise volume range in present study (table 5) is 0.036-0.07µm^3] are somewhat lower (13 to 19fg, Table5) and size-dependent."
Entered by Uri M
ID 115335