Percentages of target species in ruminal samples relative to total Eubacterial content

Range Table - link %
Organism Cow Bos Taurus
Reference Stevenson DM, Weimer PJ. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Appl Microbiol Biotechnol. 2007 May 75(1):165-74. DOI: 10.1007/s00253-006-0802-y p.170 table 4PubMed ID17235560
Method Abstract: "Relative quantification real-time PCR was used to quantify several bacterial species in ruminal samples from two lactating cows, each sampled 3 h after feeding on two successive days. Abundance of each target taxon was calculated as a fraction of the total 16S rRNA gene copies in the samples, using taxon-specific and eubacterial domain-level primers."
Comments P.170 left column 2nd paragraph: "Despite substantial differences in the productivity of the two cows and in ruminal pH (Table 1), the proportion of 16S rRNA genes in the sample that were attributable to each taxon were remarkably similar (P > 0.05) between cows and between sample days (Table 4). While there was a trend toward higher relative population size (based on proportion of 16S rRNA gene copies) of the genus Prevotella in the samples from cow 4991, this trend likewise was not statistically significant (P > 0.05). Consequently, the data from both cows on both sampling days were combined within a target taxon for comparison of population sizes across taxa. The genus-level primer for Prevotella sp. revealed a clear dominance (42% to 60% of the ruminal bacterial 16S rRNA copies) of this genus in the ruminal samples from both cows (Table 4)."
Entered by Uri M
ID 112835